Environmental DNA (eDNA) data from the 2019 and 2020 Gulf of Alaska International Year of the Salmon Expeditions

This dataset contains environmental DNA (eDNA) data collected in the Northeast Pacific Ocean. These data were collected as part of the International Year of the Salmon (IYS) Gulf of Alaska High Seas Expedition conducted in March and April 2020 and 2021, to further improve the understanding of factors impacting salmon early marine winter survival. eDNA analysis uses the free DNA shed from organisms and available in the environment to assess species diversity and composition. The eDNA database will provide overall composition of nekton, micronekton and zooplankton allowing estimation of salmon prey and potential predators. Water collected with Niskin bottle from 2-3m. 2L filtered onto Sterivex column for each replicate. Filters flash frozen. DNA extracted from filters using DNeasy kits (QUIAGEN). 16S and COI rRNA gens were amplified with PCR and sequenced on Illumina MiSeq platform using SE at 300 cycles. Reads were processed using obitools (https://pythonhosted.org/OBITools/welcome.html) and queired against nt using BLASTn. Reads were assigned to OTU using MEGAN (https://uni-tuebingen.de/fakultaeten/mathematisch-naturwissenschaftliche-fakultaet/fachbereiche/informatik/lehrstuehle/algorithms-in-bioinformatics/software/megan6/). Results were filtered using a cuttoff of >/=10 reads for positive detection. Detection of obvious contaminations belonging to human or food waste (sheep, pig, chicken, cow) as well as artificial positive controls were removed. For detailed information contact Dr. Christoph Deeg: chdeeg@mail.ubc.ca

Access and Use

Licence: Creative Commons Attribution 4.0

Data and Resources

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Keywords

Dataset extent

Map data © OpenStreetMap contributors

Metadata Reference Date(s) February 01, 2022 (Publication)
June 17, 2022 (Revision)
Dataset Reference Date(s) February 18, 2019 (Creation)
June 17, 2022 (Publication)
Frequency of Update As Needed

Responsible Party 1
Affiliation
North Pacific Anadromous Fish Commission
Email
secretariat@npafc.org
Role
Publisher
Responsible Party 2
Name
Christoph Deeg
Affiliation
Fisheries and Oceans Canada
Email
Christoph.Deeg@dfo-mpo.gc.ca
Role
  • Author
  • Custodian
  • Owner
Responsible Party 3
Name
Kristi Miller-Saunders
Affiliation
Fisheries and Oceans Canada
Email
Kristi.Saunders@dfo-mpo.gc.ca
Role
Principal Investigator
Responsible Party 4
Affiliation
Hakai Institute
Email
data@hakai.org
Role
Distributor

Field Value
Ocean Variables
  • Zooplankton biomass and diversity
  • Fish abundance and distribution
Scope Dataset
Status Completed
Maintenance Note Generated from https://cioos-siooc.github.io/metadata-entry-form
Spatial Extent { "coordinates": [ [ [ -154.1, 47.67 ], [ -124.5, 47.67 ], [ -124.5, 58.94 ], [ -154.1, 58.94 ], [ -154.1, 47.67 ] ] ], "type": "Polygon" }
North Bounding Latitude 58.94
South Bounding Latitude 47.67
East Bounding Longitude -124.5
West Bounding Longitude -154.1
Temporal Extent
Begin
2019-02-18
End
2020-04-05
Vertical Extent
Min
2.0
Max
3.0
Default Locale English
Citation identifier
Code
10.21966/2cpk-8922